ABSTRACT
The use of biocatalysts, that is, whole cells or isolated enzymes in organic synthesis, has been constantlygrowingoverthelastdecades,bothonlabscale[1-3]andindustrialscale[4,5].Enzymes showremarkablechemo-,regio-,andstereoselectivitiesandarethereforeusedinabroadrangeof resolutionreactionsorasymmetricsyntheses,forexample,inthesynthesisofchiralbuildingblocks (amines,alcohols),carbohydrateandpeptidechemistry,orothertransformations.Especiallythe improvements in molecular biology during the last decade, particularly in the šeld of protein engineering,allowedtocreatebiocatalystswithpropertiesadaptedtochemicalsynthesis,forexample, higherselectivityorprocessstability[6-8].Thishasleadtoanoverallincreaseintheapplication of enzymes for chemical purposes.
