ABSTRACT

Fluorescence and vibrational spectroscopies are traditionally used for the study of biological objects. New technical facilities like highly sensitive optical multichannel analyzers, fiber-optic systems, confocal microscopes, and Fourier transform infrared (IR) spectrometers have proceeded to an increasingly expanding area of biomedical applications of these spectroscopies. Light scattering spectroscopy provides valuable information about subsurface structure and tissue functioning. Polarization-sensitive and spectral optical coherence tomography techniques are of great interest for the future of biomedical spectroscopy. The dynamic light scattering spectroscopies are very useful both for weak scattering and for dense biological media analysis. They are very useful for noninvasive tissue and blood diagnostics. Transient techniques such as optothermal radiometry, opto-acoustics, and photo-acoustics provide a convenient means for in vivo and in situ monitoring of tissue optical and thermal properties.