ABSTRACT
Lectins, carbohydrate-binding proteins, are involved in numerous molecular recognition events associated with the immune response, tumorigenesis, and apoptosis that include cell-cell interaction and adhesion, trafˆcking of glycoproteins, and glycosylation (Sharon 2008; Sharon and Lis 1989, 2004). Unfortunately, for a large number of these important biological activities detailed mechanisms of the
2.1 Introduction ....................................................................................................29 2.2 Exchange Rate and NMR Chemical Shift Difference .................................... 31 2.3 Chemical Shift Perturbation Studies .............................................................. 32
2.3.1 Chemical Shift Mapping in the Fast Exchange Regime: Manα(1-2)Man Binding to LKAMG .................................................. 32
2.3.2 Chemical Shift Mapping in the Slow Exchange Regime: Hexaacetyl Chitohexaose Binding to MoCVNH-LysM ..................... 33
2.4 Saturation Transfer Difference NMR ............................................................. 35 2.4.1 Mapping of the CV-N-Binding Epitope on Oligomannosides
by STD-NMR .....................................................................................36 2.5 Transferred Nuclear Overhauser Effect .......................................................... 38
2.5.1 The Conformation of the Tisaccharide Rhaα(1-2)[GlcNAcβ(1-3)] Rhaα(1-OPr) Bound to a Monoclonal Antibody ................................ 39
2.6 Determination of Carbohydrate-Lectin Complex Structures by Standard NOESY Experiments ......................................................................40 2.6.1 The Structure of the CV-N-Manα(1-2)Man Complex ........................40
