ABSTRACT
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8.1 Introduction Recent advances in vibrational imaging based on coherent Raman scattering (CRS) are opening up exciting opportunities for dynamic, non-invasive, and compositional imaging. In CRS microscopy strong signals are generated by focusing picosecond (ps) or femtosecond (fs) excitation laser power on a single Raman mode, which allows imaging at video rate speed (Evans et al., 2005; Saar et al., 2010). In addition same excitation lasers can be used for multiphoton imaging on the same platform (for a review see Yue et al. [2011b]).
