ABSTRACT
As discussed in Chapter 4, stimulated Raman scattering (SRS) microscopy allows fast, threedimensional imaging with chemical contrast based on intrinsic spectroscopic properties of the sample (Ploetz et al. 2007, Freudiger et al. 2008, Ozeki et al. 2009, Nandakumar et al. 2009, Saar et al. 2010a). It has superseded the older coherent Raman microscopy technique, coherent anti-Stokes Raman scattering (CARS) in that
• SRS is free from non-resonant background • SRS has much improved sensitivity • SRS does not su—er from spectral distortions • SRS signal is linear in the concentration of the target species • SRS does not have imaging artifacts due to phase-matching
is chapter summarizes important applications of SRS microscopy that capitalize on both high sensitivity and straightforward quanti cation of SRS. While CARS microscopy has o‚en been criticized as being mainly useful for lipid imaging, SRS has allowed us to image a variety of biomolecules. Table 24.1 summarizes the growing number of molecular species that have been imaged with SRS microscopy thus far.
