ABSTRACT
Keywords .............................................................................................. 122 References ............................................................................................. 122
6.1 CLONING OF MULTI-COPY CPG MOTIFS IN BACTERIAL PLASMIDS
6.1.1 INTRODUCTION
Two plasmids with different copy number of unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs) were constructed. The plasmid pCpG-KH11 carries 96 copies of CpG motif GTCGTT, with latter being the most effective in stimulation of the human immune system in accordance with literature data. The plasmid pCpG-KM11 contains 120 repetitions of GACGTT-motif found to enhance immune effects in mice, fish, and so on. The sequence of inserted fragments was verified by PCR and restriction enzyme digestion.
