ABSTRACT
Gradient elution in liquid chromatography is a powerful technique based on programmed separation modes; therefore, the operation conditions are changed according to a preset program during the elution [1-6]. The main purpose of gradient elution
3.1 Introduction .................................................................................................... 79 3.2 Basic Relationships ......................................................................................... 81
3.2.1 Multilinear Gradient Profiles .............................................................. 81 3.2.2 The Fundamental Equation of Gradient Elution ................................ 82 3.2.3 Retention Models ................................................................................ 83
3.3 Fitting and Optimization Approaches ............................................................84 3.3.1 Fitting Retention Models ....................................................................85
