ABSTRACT
Peak Shapes, and Retention Times ...................................................208 5.6.3 Estimation of the Equilibrium Constant ........................................... 210 5.6.4 Summary .......................................................................................... 211
5.7 Case IV(B): Reversible Dimerization of a Protein in Immobilized Metal Affinity Chromatography ............................................................................. 211 5.7.1 System Considered and Model Testing ............................................. 211 5.7.2 Effect of Adsorption Isotherm, Nk+, Nk-, and Nk+/Nk-on the
Breakthrough Curves ........................................................................ 213 5.7.3 Dynamic Binding Capacity .............................................................. 215 5.7.4 Column Dynamics ............................................................................ 219 5.7.5 Summary ..........................................................................................220
