ABSTRACT
I. INTRODUCTION Electrophoresis as an analytical technique was first introduced by Tiselius in 1930 [1]. In his thesis he described the separation of blood plasma proteins, namely albumin from a, p and yglobulin, using electrophoresis. For this pioneering work, Tiselius was awarded the Nobel Prize in 1948. Since then, many advancements in electrophoresis, such as paper and gel electrophoresis, have been introduced. Filter paper has been widely used as a supporting medium since the late 1940s for the separation of ionized compounds such as amino acids, lipids, nucleotides, and charged sugars [2]. Two-dimensional paper electrophoresis was carried out to enhance the resolution by changing the buffer's pH, i.e., first-dimension electrophoresis at a particular pH and second-dimension at a different pH.
