ABSTRACT
Figure 7 Microsomal retinal synthesis from unbound retinol. Retinal generated in the control reactions run in !he absence of cofactor circles) was subtracted from the retinal observed in the presence of 2 mM NAD retinal, filled to provide the cofactor-dependent rate of retinal production retinal, triangles). The kinetic values for this specific experiment, as determined by nonlinear regression analyses, were: for gross retinal, Km = 76 !J.M and Vmax = 475 pmol/min/mg protein; for net retinal, Km = 20 !J.M and Vmax = 124 pmol/min/mg protein.
