with 10:1 ratio. The regenerated stigmas were biologically mature and were able to receive pollen. Recently, explants from halved immature ovaries were used in different base medias with different proportion of growth regulators to evaluate frequency of formation of stigmas (58). Results showed that after 50 to 60 days structures similar to stigma were successfully formed in B5 medium. The medium contained NAA (5.43 p.m), BA (44 ilm), organic compounds of MS medium, hydrolyzed cazeine (5%) and L-alanine (11.2 inn).

doi:10.1201/9781482280463-17

Chapter

with 10:1 ratio. The regenerated stigmas were biologically mature and were able to receive pollen. Recently, explants from halved immature ovaries were used in different base medias with different proportion of growth regulators to evaluate frequency of formation of stigmas (58). Results showed that after 50 to 60 days structures similar to stigma were successfully formed in B5 medium. The medium contained NAA (5.43 p.m), BA (44 ilm), organic compounds of MS medium, hydrolyzed cazeine (5%) and L-alanine (11.2 inn).

Chapter

with 10:1 ratio. The regenerated stigmas were biologically mature and were able to receive pollen. Recently, explants from halved immature ovaries were used in different base medias with different proportion of growth regulators to evaluate frequency of formation of stigmas (58). Results showed that after 50 to 60 days structures similar to stigma were successfully formed in B5 medium. The medium contained NAA (5.43 p.m), BA (44 ilm), organic compounds of MS medium, hydrolyzed cazeine (5%) and L-alanine (11.2 inn).

ABSTRACT

In recent years in vitro synthesis of some saffron metabolites and their biochemical conversion have been studied. As an example, conjugated sugars with anti-cancer effects has been isolated from callus culture of saffron corms (33). Biochemical pathways for metabolites such as crocetin monoglucosyl and diglucobil in saffron tissue cultures have been studied using electrophoresis method. These results are the basis for production of synthetic compounds similar to saffron metabolites (36, 37).