ABSTRACT

Figure 2.12 demonstrates the fluorescence spectra of the pistil stigma from various plant species. Stigmas which were studied have one or two maxima of their spectra: one - in Aesculus hippocastanum, Berberis vulgaris or Hemerocallis fulva - (525, 560 or 475 nm, respectively), and two in Petunia hybrida (520 and 680 nm). It is clearly seen that the stigma pellicles may contain chlorophyll fluoresced with maximum 680 nm or not. While in pollen grain emission, numerous components are found. There are three maxima in both A esculus hippocastanum and Petunia hybrida (500, 580, 620 and 450, 515, 650 nm, respectively). Pollen of Berberis vulgaris has two maxima at 525 and 645 nm, whereas pollen of Hemerocallis fulva has only one 565 nm maximum. Both the spectra forms and the maxima positions of the pistil fluorescence differ from those of the pollen grains. The difference depends on the nature of the species and the composition of the pollen grain surface. Blue fluorescence of pistils was also demonstrated earlier by Kendrick and Knox (1981). The observed changes in