ABSTRACT
In1980,thefirstmonoclonalbloodgroupantibodyusedinbloodgrouping-ananti-A-was described(37).Duringthenextfewyears,anti-Bandanti-A+Bmonoclonalantibodieswere produced(38-43).Theantibodieswereraisedinmice,andhumanRBCsornaturalorsynthetic bloodgroup-activesubstanceswereusedfortheimmunization.Mostoftheseantibodiesare oftheIgMtypeandhencegoodagglutinators,butstronglyagglutinatinglgA(22)andIgG antibodiesalsoexist.ThefirstoftheseMAbreagentswereofnearlythesamequalityasthe currentlyavailablehumanpolyclonalserum-basedreagentsoronlyslightlybetter.Soonsuperior MAbsweremadeandformulatedintoreagents.Anti-AMAbsweremadethatreadilydetected evenveryweakvariantsofA,suchAsAx,andweresuperiorinperformancetohumanreagents. In1987,theISBTarrangedanInternationalWorkshoponMonoclonalAntibodies.Manyof theABOMAbsthenavailableweretestedinlaboratoriesworldwide,anditwasconcludedthat someoftheseMAbs,eitheraloneorblendedwithsuitableotherMAbs,wereexcellentreagents thatweresuperiortopolyclonalreagents.Atthattime,nosatisfactorysingleanti-A+BMAb wasinexistence,butsuchMAbsantibodieshavebeenproducedsince,andthepossibilityto useasingleMAbforsuchareagentwasclearlyshowninthesecondISBTInternational WorkshoponMonoclonalAntibodies(44).Itwasshownattheseworkshopsthatthebest reagentsweremadefromMAbsthatreactedwithallvariantsoftheAstructure(foranti-A)or theBstructure(foranti-B).ThosemonoclonalsthatonlyreactedwithparticulartypesofAor Bstructuregenerallyperformedpoorlyasreagents.
