ABSTRACT
Complexants other than alcohols (e.g., amines, nitriles, tert-butyl com pounds, surfactants, and multiple fluorophores) can also be detected by the method of Scheme 2 [279-284]. Bohne and Yang have replaced alcohols with amino acids [285], although the zwitterionic charge distribution and increased steric requirements of the former lead to smaller stability constants of the ternary complex. Notwithstanding, tryptophan, leucine, and phenylalanine produce mea surable changes in the I:III ratio of pyrene emission, thereby allowing for their detection at mM levels.
