ABSTRACT
SECTION 1 RESTRICTION MAPPING 56 Part I Introduction 56
1. What Is Restriction Mapping? 58 2. Why Is Restriction Mapping Useful? 58 3. Online Restriction Mapping Programs 59
Part II Step-By-Step Tutorial 61 1. Input Your Genomic or cDNA Sequence 61 2. Results: Display and Analysis 62
Part III Sample Data 66 SECTION 2 PCR APPLICATION 67
Part I Introduction 67 1. How PCR Works 67 2. Quantitative Real-Time PCR 68 3. PCR Applications 68 4. PCR Primer Design 70
Part II Step-By-Step Tutorial 72 1. Sequence Input and Parameters Selection 72 2. Results 75
Part III Sample Data 76
The discovery of restriction enzymes from microorganisms ushered in the era of recombinant DNA technology and the technologies of genetic cloning and genetic engineering during the second half of the twentieth century. Similarly, the polymerase chain reaction (PCR) methodology facilitated unexpected breakthroughs in many life science disciplines, including clinical and basic research. Progress will certainly continue as more robust and faster computational tools emerge and continue to improve. This chapter focuses on the application of bioinformatics tools to restriction mapping and PCR, two of the most common DNA analyses.
