ABSTRACT
Growing cells using three-dimensional cell culture models is known
to radically enhance cell functionality over conventional systems
in which cells adapt abnormally to the two-dimensional growth
substrate. This review article briefly considers some of the current
technologies developed to enable three-dimensional cell growth in
vitro and focuses on the practical issues surrounding their ability for
routine use in cell culture laboratory. A simple solution for routine
three-dimensional culture known as Alvetex R© is highlighted, and methods for its use and application are described.
