ABSTRACT
In vitro assessment of inhalation toxicity is an emerging and fast-
growing field. Until now, only animal models were used in OECD
test guidelines for inhalation toxicity testing, and therefore in vitro
alternatives of human origin are urgently needed. In the present
chapter, we first review the toolbox of in vitro human airwaymodels
currently available. We then focus on the applications of the human
airway epithelia (MucilAirTM), a 3D cell model with a long shelf life,
which is used for acute, long-term, and repeated dose testing. A
panel of tests relevant for inhalation toxicology is described.
