Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

part I|7 pages


chapter one|5 pages

Introduction to plant tissue culture

ByDennis J. Gray, Robert N. Trigiano

part II|9 pages

History of plant tissue culture

chapter two|7 pages

History of plant tissue and cell culture

ByJames D. Caponetti, Dennis J. Gray, Robert N. Trigiano

part III|54 pages

Supporting methodologies

chapter four|6 pages

Nutrition of callus cultures

ByJames D. Caponetti

chapter five|9 pages

Histological techniques

ByRobert N. Trigiano, Kathleen R. Malueg, Effin T. Graham

chapter six|6 pages

Photographic methods for plant cell and tissue culture*

ByDennis J. Gray

chapter seven|12 pages

Statistical analysis of plant tissue culture data

ByMichael E. Compton

part IV|174 pages

Propagation techniques

chapter eight|12 pages

Propagation from preexisting meristems

ByMichael E. Kane

chapter nine|9 pages

Micropropagation of Syngonium by shoot culture

ByMichael E. Kane

chapter ten|6 pages

Micropropagation of Dieffenbachia

ByR. J. Henny, Lee Goode, Wanda Ellis

chapter eleven|8 pages

Micropropagation of potato by node culture and microtuber production *

ByMichael E. Kane

chapter twelve|8 pages

Micropropagation of lilacs

ByDeborah D. McCown, Andrew J. Daun

chapter thirteen|6 pages

Micropropagation and in vitro flowering of rose

ByMichael E. Kane

chapter fourteen|14 pages


ByOtto J. Schwarz, Robert M. Beaty

chapter fifteen|10 pages

Direct shoot organogenesis from leaf explants of chrysanthemum

ByRobert N. Trigiano, Roger A. May

chapter sixteen|10 pages

Shoot organogenesis from cotyledon explants of watermelon

ByMichael E. Compton, Dennis J. Gray

chapter seventeen|8 pages

Direct and indirect shoot organogenesis from leaves of Torenia fournieri

ByMark P. Bridgen

chapter eighteen|8 pages

Shoot organogenesis from petunia leaves

ByJohn E. Preece

chapter nineteen|16 pages

Nonzygotic embryogenesis *

ByDennis J. Gray

chapter twenty|14 pages

Embryogenic callus and suspension cultures from leaves of orchardgrass *

ByDennis J. Gray, Robert N. Trigiano, Bob V. Conger

chapter twenty-one|7 pages

Somatic embryogenesis from seeds of melon*

ByDennis J. Gray

chapter twenty-two|9 pages

Somatic embryogenesis from mature peanut seed

ByHazel Y. Wetzstein, Charleen Baker

chapter twenty-three|7 pages

Direct somatic embryogenesis from leaves and flower receptacles of cineraria

ByRobert N. Trigiano, Mary Catherine Scott, Kathleen R. Malueg

chapter twenty-four|8 pages

Somatic embryogenesis from immature seeds of yellow poplar

ByScott A. Merkle

chapter twenty-five|8 pages

Induction of somatic embryogenesis in conifers

ByStephen M. Attree, Patricia J. Rennie, Larry C. Fowke

part V|137 pages

Crop improvement techniques

chapter twenty-six|13 pages

Use of protoplasts for plant improvement

ByMichael E. Compton, James A. Saunders, Richard E. Veilleux

chapter twenty-seven|14 pages

Isolation, culture, and fusion of tobacco and potato protoplasts

ByRichard E. Veilleux, Michael E. Compton

chapter twenty-eight|8 pages

Isolation of protoplasts from leaves of chrysanthemum and orchardgrass

ByRobert N. Trigiano

chapter twenty-nine|6 pages

Haploid cultures

BySandra M. Reed

chapter thirty|6 pages

Production of haploid tobacco plants using anther culture

BySandra M. Reed

chapter thirty-one|7 pages


ByMelina López-Meyer, Ignacio E. Maldonado-Mendoza, Craig L. Nessler

chapter thirty-two|16 pages

Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the β-glucuronidase (GUS) reporter gene

ByIgnacio E. Maldonado-Mendoza, Melina López-Meyer, Craig L. Nessler

chapter thirty-three|8 pages

Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens

ByRobert N. Trigiano

chapter thirty-four|8 pages

Construction and use of a simple gene gun for particle bombardment*

ByDennis J. Gray, Michael E. Compton, Ernest Hiebert, Chia-Min Lin, Victor P. Gaba

chapter thirty-five|8 pages

Germplasm preservation

ByLeigh E. Towill

chapter thirty-six|10 pages

Vitrification as a method to cryopreserve shoot tips

ByLeigh E. Towill

chapter thirty-seven|6 pages

Secondary product expression in vitro

ByMary Ann L. Smith

chapter thirty-eight|12 pages

Pigment production in Ajuga cell cultures

ByMary Ann L. Smith, Randy B. Rogers

chapter thirty-nine|12 pages

In vitro plant pathology

ByC. Jacyn Baker, Norton M. Mock, Elizabeth W. Orlandi

part VI|47 pages

Special topics

chapter forty|10 pages

Variation in tissue culture

ByS. Jayasankar

chapter forty-one|9 pages

Short-circuiting the fern life cycle: apospory in Ceratopteris richardii

ByLeslie G. Hickok, Thomas R. Warne

chapter forty-two|10 pages

Commercial laboratory production

ByGayle R. L. Suttle

chapter forty-three|9 pages

Indexing for plant pathogens

ByMichael J. Klopmeyer

chapter forty-four|6 pages

Culture indexing for bacterial and fungal contaminants

ByMichael E. Kane