Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.

part 1|96 pages

Biophysical Methods

chapter 1|12 pages

X-Ray Microanalysis

ByW. M. Hess, C. Z. Hess, M. D. Standing

chapter 2|15 pages

Immunoelectron Microscopy

ByNicole Benhamou, Richard Bélanger

chapter 3|21 pages

Diagnosing Plant Virus Diseases by Light Microscopy *

ByR. G. Christie, J. R. Edwardson, G. W. Simone

chapter 4|8 pages

Use of DNA-Binding Fluorochromes for the Nuclear Staining in Fungi

ByUma S. Singh, J. Kumar, Amita Sachan, Pratibha Singh

chapter 5|8 pages

Virion Gel Electrophoresis

ByRudra P. Singh, Norman R. M. Watts

chapter 6|11 pages

On the Mechanism of Viroid Strain Separation in Gel Electrophoresis

ByNorman R. M. Watts, Rudra P. Singh

chapter 7|16 pages

Electrophoretic Karyotyping: Method and Applications

ByDallice Mills, Kevin McCluskey, Brian W. Russell, Jacqueline Agnan

part 2|34 pages

Biophysical Methods

chapter 8|16 pages

Biochemical Markers for Disease Resistance *

ByReuven Reuveni

chapter 9|16 pages

Isozymes: Methods and Applications *

ByJ. A. Micales, M. R. Bonde

part 3|140 pages

Biomolecular Methods

chapter 11|5 pages

Tissue-Print Hybridization for the Detection and Localization of Plant Viruses

ByTet-Fatt Chia, Yang-Sun Chan, Nam-Hai Chua

chapter 12|15 pages

Transposon and Marker Exchange Mutagenesis

ByGeorge H. Lacy, Verlyn K. Stromberg

chapter 13|21 pages

Polymerase Chain Reaction Technology in Plant Pathology

ByA. Hadidi, L. Levy, E. V. Podleckis

chapter 14|14 pages

Gene Transfer to Higher Plants

ByRalf R. Mendel, Robert M. Hänsch

chapter 15|17 pages

Arabidopsis as a Model Host in Molecular Plant Pathology

ByRoger Innes

chapter 16|14 pages

Isolation and Cloning of Plant Disease Resistance Genes

ByNevin Dale Young

chapter 17|14 pages

Molecular Strategies to Develop Virus-Resistant Plants

ByJames N. Culver

chapter 18|22 pages

Engineering Disease Resistance in Plants: An Overview

ByRichard A. Dixon, Nancy L. Paiva, Madan K. Bhattacharyya

part 4|233 pages

Biotechnological Methods

chapter 19|15 pages

Present Problems in and Aspects of Breeding for Disease Resistance

ByJ. E. Parlevliet

chapter 20|12 pages

Dual Culture: Fungi

ByO. K. Sinha

chapter 21|12 pages

Dual Culture: Nematodes

ByS. Verdejo-Lucas

chapter 22|15 pages

Protoplast Culture: Viruses and Viroids *

ByAbed Gera

chapter 23|13 pages

ELISA Methodology

ByR. K. Khetarpal, C. A. Kumar

chapter 24|16 pages

Methods for the Production of Mouse Monoclonal Antibodies

ByR. Mernaugh, G. Mernaugh

chapter 25|7 pages

An Overview of Phage-Displayed Recombinant Antibodies

ByR. Mernaugh, G. Mernaugh

chapter 26|10 pages

Direct Tissue Blot Immunoassay for Analysis of Plant Pathogens *

ByH. T. Hsu, R. H. Lawson, N. S. Lin, Y. H. Hsu

chapter 27|17 pages

Monoclonal Antibodies to Fungal Plant Pathogens: Basic and Applied Aspects

ByKeith L. Wycoff, Laurence M. Brill

chapter 29|18 pages

Monoclonal Antibodies: Viruses

ByKazusato Ohshima

chapter 30|13 pages

Monoclonal Antibodies: Nematodes

ByJohn M. S. Forrest

chapter 31|16 pages

Immunochemical Methods for Detection of Toxins and Pesticides

ByTseng Tsung-Che

chapter 32|13 pages

Plant Disease Diagnosis: Biotechnological Approaches

BySally A. Miller