Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction.
G Protein-Coupled Receptors has culled contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the r

section Section I

Novel Ligands: Ligand Screening Systems and Orphan Receptors

chapter 1|48 pages

Measurement of Competitive and Allosteric Interactions in Radioligand Binding Studies

BySebastian Lazareno, Nigel J.M. Birdsall

chapter 2|22 pages

Ligand Screening of G Protein-Coupled Receptors in Yeast

ByJohn R. Hadcock, Mark H. Pausch

chapter 3|26 pages

Ligand Screening Using Melanophore Cells: Frog Skin to Combinatorial Chemistry

ByJ. Mark Quillan, Mac E. Hadley

chapter 4|18 pages

Screening Orphan Receptors for Native Ligands

ByShelagh Wilson, J. Randall Slemmon, Jeffrey S. Culp, Brian D. Hellmig, Dean E. McNulty, Robert S. Ames, Henry M. Sarau, James J. Foley, Janet E. Park, Jon K. Chambers, Alison I. Muir, Jeffrey M. Stadel, Derk J. Bergsma

section Section II|2 pages

Function and Regulation: Receptor–G Protein Coupling and Desensitization

chapter 5|38 pages

Reconstitution of Receptor-Promoted Signal Transduction Using Purified Receptor, G Protein, and Effector

ByGabriel Berstein, Gloria H. Biddlecome

chapter 6|30 pages

Mutagenesis Approaches for Studying Receptor-G Protein Interactions and Receptor Assembly

ByFu-Yue Zeng, Evi Kostenis, Jan Jakubik, Xiangyang Zhu, Torsten Schöneberg, Jürgen Wess

chapter 7|26 pages

G Protein-Coupled Receptor Kinases and Desensitization of Receptors

ByHitoshi Kurose

chapter 8|28 pages

Internalization and Downregulation of G Protein-Coupled Receptors

ByJelveh Lameh

chapter 9|24 pages

Use of Electrophysiology to Monitor and Study Receptor Desensitization

ByMark R. Boyett, Zhigang Shui, Iftikhar A. Khan

section Section III|1 pages

3D Structure: Large-Scale Expression and Electron- and X-Ray Crystallography

chapter 10|16 pages

Expression in Escherichia coli and Large-Scale Purification of a Rat Neurotensin Receptor

ByReinhard Grisshammer, Julie Tucker

chapter 11|42 pages

Large-Scale Expression of Receptors in Yeasts

ByHelmut Reiländer, Christoph Reinhart, Agnes Szmolenszky

chapter 12|32 pages

Electron Crystallography of Membrane Proteins

ByYoshinori Fujiyoshi

chapter 14|21 pages

X-Ray Crystallography of Membrane Proteins: Concepts and Applications of Lipidic Mesophases to Three-Dimensional Membrane Protein Crystallization

ByMichele Loewen, Mark L. Chiu, Christine Widmer, Ehud M. Landau, Jurg P. Rosenbusch, Peter Nollert