Arousal states are processes that include waking, deep sleep, and the dreaming phase of sleep (REM). Molecular Regulation of Arousal States explores the cellular and molecular mechanisms by which sleep and wakefulness are regulated and seeks explanations for the generation of arousal states. It presents step-by-step research protocols that allow investigators to apply the techniques described to a wide range of physiological and behavioral research problems, such as sleep neurobiology and state-dependent disruption of cardiopulmonary control. For the first time, a single source integrates cellular and molecular research techniques with studies of arousal, opening the door to exciting new research methodologies.

chapter Chapter 1|10 pages

Application of In Situ Hybridization to the Study of Rhythmic Neural Systems

ByH. Elliott Albers, R. Thomas Zoeller, Kim L. Huhman

chapter Chapter 3|10 pages

Voltammetric Detection of Nitric Oxide (NO) in the Rat Brain: Release Throughout the Sleep–Wake Cycle

BySophie Burlet, Raymond Cespuglio

chapter Chapter 4|12 pages

Sleep Regulatory Substances: Change in mRNA Expression Linked to Sleep

ByZutang Chen, James M. Krueger

chapter Chapter 6|13 pages

Methods for the Measurement of Adenylyl Cyclase Activity

ByCharles W. Emala

chapter Chapter 7|19 pages

Methods Used to Assess Specific Messenger RNA Expression During Sleep

ByMary Ann Greco, Lalini Ramanathan, Radhika Basheer, Priyattam J. Shiromani

chapter Chapter 9|16 pages

Isolation and Identification of Specific Transcripts by Subtractive Hybridization

ByThomas S. Kilduff, Luis de Lecea, Hiroshi Usui, J. Gregor Sutcliffe

chapter Chapter 11|12 pages

Transcriptional Regulation of Putative Sleep-Promoting Compounds

ByMiroslaw Mackiewicz, Sigrid C. Veasey, Allan I. Pack

chapter Chapter 12|14 pages

Chemical Mutagenesis and Screening for Mouse Mutations with an Altered Rest–Activity Pattern

ByPatrick M. Nolan, Thomas A. Houpt, Maja Bucan

chapter Chapter 14|13 pages

In Situ Hybridization of Messenger RNA in Sleep Research

ByTarja Porkka-Heiskanen, Jussi Toppila, Dag Stenberg

chapter Chapter 15|10 pages

New Directions in the Analysis of Brain Substances Related to Sleep and Wakefulness

ByGary Siuzdak, Steven Henriksen

chapter Chapter 16|9 pages

Sleep and Circadian Rest–Activity Rhythms in Prion Protein Knockout Mice

ByIrene M. Tobler, Marek Fischer, Jean C. Manson

chapter Chapter 17|12 pages

Measurement of Nitric Oxide in the Brain Using the Hemoglobin Trapping Technique Coupled with In Vivo Microdialysis

ByJulie A. Williams, Steven R. Vincent, Peter B. Reiner

chapter Chapter 18|8 pages

Mapping Regional Cerebral Protein Synthesis During Sleep

ByRebecca K. Zoltoski