This book is a comprehensive compilation of modern and cutting-edge chromatographic techniques written by pharmaceutical industry experts, academics, and vendors in the field. This book is an inclusive guide to developing all chromatographic methods

(such as liquid chromatography and gas chromatography). It covers modern techniques for developing methods using chromatographic development software, requirements for validations, discussion on orthogonality, and how to transfer methods from HPLC to UHPLC. The text introduces some newer techniques that are heavily employed by chemists analyzing proteins and RNAi, as well as novel techniques such as counter current chromatography. This book is valuable for both the novice starting out in undergraduate labs and those who are new to the pharmaceutical industry and is a useful reference for seasoned analysts.  

chapter Chapter 1|11 pages

A Lesson Learned from UHPLC

ByGregory K. Webster, Laila Kott

chapter Chapter 2|40 pages

Software for Chromatographic Methods Development

ByAoi Ariyasu, Eiki Maeda

chapter Chapter 3|12 pages

Flash Chromatography Method Development

ByJ. Robert Bickler

chapter Chapter 4|20 pages

Orthogonality in Chromatographic Methods

ByZachary S. Breitbach, Gregory K. Webster

chapter Chapter 5|30 pages

Unified Approach to Reversed-Phase and Normal-Phase Chromatographic Method Development

ByGregory K. Webster

chapter Chapter 6|41 pages

Polysaccharide-Derived Chiral Stationary Phases for the Separation of Enantiomers

ByTong Zhang, Pilar Franco

chapter Chapter 7|22 pages

Chiral Chromatography: Method Development

ByLaila Kott, Gregory K. Webster

chapter Chapter 8|30 pages

Aqueous Normal-Phase Chromatography Using Type C Silica Columns

ByJoseph J. Pesek, Maria T. Matyska

chapter Chapter 9|28 pages

Ion Chromatography Columns

ByChristopher Pohl

chapter Chapter 10|25 pages

Ion Chromatography: Method Development

ByLaila Kott

chapter Chapter 11|46 pages

Fundamentals, Properties, and Applications of Stationary Phases for Gas Chromatography Method Development

ByOmprakash Nacham, Jared L. Anderson

chapter Chapter 12|58 pages

Gas Chromatography Method Development

ByTien Ho, John C. Vinci

chapter Chapter 13|30 pages

Method Transfer between HPLC and UHPLC

BySusanne Fabel

chapter Chapter 14|18 pages

Small-Molecule Pharmaceutical Impurities Test Method Validation: Precision Acceptance Criterion

ByRussell L. Hertzler, Mark D. Johnson, Teodora Moldovan, Michael D. Oberlander, James Reynolds, Holger van Lishaut, Yanbing Zheng

chapter Chapter 16|25 pages

Size Exclusion Chromatography Method Development for Therapeutic Proteins

ByAlexandre Goyon, Jean-Luc Veuthey, Davy Guillarme, Szabolcs Fekete