While silk derived from silkworm has been of economic importance for centuries, more recently silkworm has been found to have utility in biomedicine. This has attracted attention for expressing eukaryotic recombinant proteins, which require post-translational modifications. In 1985 Dr. Susumu Maeda demonstrated that silkworm larvae could produce a functional human-interferon. Since then various techniques have been developed to express recombinant proteins in silkworm. With the development of the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system, which is capable of replicating in both Escherichia coli and Bombyx mori derived cell lines or silkworm, silkworm larvae or pupae have been used for the expression system for recombinant protein production. This method has the advantage of a bacmid, in that it can be easily prepared for sufficient bacmid DNA for subsequent expression in silkworm. It is potentially a big breakthrough in production of recombinant eukaryotic proteins and viruses, which will be a powerful tool in a new proteome era.

This volume contributes to the advancement of our knowledge in the subject, for example gene expression systems and silkworm research, and focuses on silkworm biofactories for the recombinant protein production and commercial applications of proteins.

part 1|2 pages

Silkworm Biology and Physiology

chapter 1|15 pages

Basic and Applied Genetics of Silkworms

ByToru Shimada

chapter 2|20 pages

Silkworm Breeding

ByYuyin Chen

chapter 3|24 pages

Focus on Physiological Vital Proteins from Silkworm Bombyx mori

ByRaman Chandrasekar, S. K. M. Habeeb

chapter 4|26 pages

Metabolism of Molting Hormone and Juvenile Hormone in Silkworm

ByYungen Miao

chapter 5|19 pages

Post-translational Modification in Silkworm

BySungjo Park, Enoch Y. Park, Andre Terzic

chapter 6|20 pages

Glycoprotein Biosynthesis of Silkworm

ByKazuhito Fujiyama

part 2|2 pages

Utilization of Silkworm as a Biofactory

chapter 7|14 pages

BacMam System in Silkworm

ByTakashi Tadokoro

chapter 8|20 pages

Transgenic Silkworm

ByHideki Sezutsu

chapter 9|17 pages

Glycosyltransferase Expression in Silkworm and its Applications in Glycobiology

ByMakoto Ogata, Taichi Usui, Enoch Y. Park

chapter 10|13 pages

Silkworm Expression of Cell Surface Molecules

ByShunsuke Kita

chapter 12|16 pages

Virus-like Particles Expression in Silkworms

ByVipin Kumar Deo, Enoch Y. Park

chapter 13|13 pages

Gene Delivery Based on Bombyx mori Nucleopolyhedrovirus (BmNPV)

ByTatsuya Kato, Enoch Y. Park

part 3|2 pages

Bioproducts from Silkworm

chapter 14|16 pages

Development of New Biomaterials from Insect and its Virus

ByEiji Kotani, Hajime Mori

chapter 15|15 pages

Production of Medical and Cosmetic Products using Transgenic Silkworms

ByMasahiro Tomita

chapter 16|21 pages

Comparative Investigation of Influenza Virus-like Particles from Synthetic Chemical Substance and Silkworm

ByKuniaki Nerome, Kazumichi Kuroda, Shigeo Sugita

chapter 17|12 pages

Production of Feline and Canine Interferon from Silkworm

ByTsuyoshi Tanaka, Takashi Tanaka