ABSTRACT

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

chapter 1|16 pages

Insect Cell Culture Engineering: An Overview

ByMattheus F. A. Goosen

chapter 2|34 pages

An Overview of the Molecular Biology and Applications of Baculoviruses

ByJohn Kuzio, Peter Faulkner

chapter 3|18 pages

Bioreactor Design for Insect Cell Cultivation: A Review

ByJanusz J. Malinowski, Andrew J. Daugulis

chapter 4|35 pages

Insect Cell Immobilization

ByMattheus F. A. Goosen

chapter 5|34 pages

Applications of Insect Cell Gene Expression in Pharmaceutical Research

ByDarrell R. Thomsen, Annette L. Meyer, Leonard E. Post

chapter 6|39 pages

Scale-Up Considerations and Bioreactor Development for Animal Cell Cultivation

ByJohannes Tramper, Kees D. de Gooijer, Just M. Vlak

chapter 7|16 pages

Serum-Free Media

ByStefan A. Weiss, Glenn P. Godwin, Stephen F. Gorfien, William G. Whitford

chapter 8|25 pages

Foreign Gene Expression in Insect Cells

ByDonald L. Jarvis

chapter 9|20 pages

The Future of Insect Cell Culture Engineering

BySpiros N. Agathos