Practical Handbook of Microbiology, 4th edition provides basic, clear and concise knowledge and practical information about working with microorganisms. Useful to anyone interested in microbes, the book is intended to especially benefit four groups: trained microbiologists working within one specific area of microbiology; people with training in other disciplines, and use microorganisms as a tool or "chemical reagent"; business people evaluating investments in microbiology focused companies; and an emerging group, people in occupations and trades that might have limited training in microbiology, but who require specific practical information.

Key Features

  • Provides a comprehensive compendium of basic information on microorganisms—from classical microbiology to genomics.
  • Includes coverage of disease-causing bacteria, bacterial viruses (phage), and the use of phage for treating diseases, and added coverage of extremophiles.
  • Features comprehensive coverage of antimicrobial agents, including chapters on anti-fungals and anti-virals.
  • Covers the Microbiome, gene editing with CRISPR, Parasites, Fungi, and Animal Viruses.
  • Adds numerous chapters especially intended for professionals such as healthcare and industrial professionals, environmental scientists and ecologists, teachers, and businesspeople.
  • Includes comprehensive survey table of Clinical, Commercial, and Research-Model bacteria.


The Open Access version of this book, available at https://www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license.

Chapter 21, "Archaea," of this book is freely available as a downloadable Open Access PDF under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license available at https://www.taylorfrancis.com

See Emanuel Goldman's Open Access article: "Lamarck redux and other false arguments against SARS-CoV-2 vaccination," https://www.embopress.org/doi/full/10.15252/embr.202254675

part Part I|158 pages

Practical Information and Procedures

chapter 21|16 pages

Sterilization, Disinfection, and Antisepsis

ByMichael G. Schmidt

chapter 2|18 pages

Quantitation of Microorganisms

ByBrad A. Slominski, Peter S. Lee

chapter 3|4 pages

Culturing and Preserving Microorganisms

ByLorrence H. Green

chapter 4|10 pages

Stains for Light Microscopy

ByStuart Chaskes, Rita Austin

chapter 5|8 pages

Identification of Gram-Positive Organisms

ByPeter M. Colaninno

chapter 6|12 pages

Identification of Aerobic Gram-Negative Bacteria

ByDonna J. Kohlerschmidt, Lisa A. Mingle, Nellie B. Dumas, Geetha Nattanmai

chapter 7|4 pages

Plaque Assay for Bacteriophage

ByEmanuel Goldman

chapter 8|14 pages

Phage Identification of Bacteria

ByCatherine E.D. Rees, Martin J. Loessner

chapter 9|14 pages

Phage Display and Selection of Protein Ligands

ByGeir Åge Løset, Wlodek Mandecki, Inger Sandlie

chapter 10|12 pages

Diagnostic Medical Microbiology

ByLorrence H. Green

chapter 11|4 pages

Modern Diagnostic Methods in the 21st Century

ByLorrence H. Green, Alan C. Ward

chapter 12|10 pages

Antibiotic Susceptibility Testing

ByAudrey Wanger, Violeta Chávez

chapter 13|6 pages

Bacterial Cell Breakage or Lysis

ByMatthew E. Bahamonde

chapter 14|2 pages

Major Culture Collections and Sources

ByLorrence H. Green

chapter 15|10 pages

Epidemiological Methods in Microbiology

ByTyler S. Brown, Barun Mathema, D. Ashley Robinson

chapter 16|12 pages


ByTao Xu, Megan L. Kempher, Xuanyu Tao, Aifen Zhou, Jizhong Zhou

part Part II|586 pages

Survey of Microorganisms

chapter 16017|6 pages

Taxonomic Classification of Bacteria

ByJ. Michael Janda

chapter 18|38 pages

Bacterial Cell Wall: Morphology and Biochemistry

ByStefania De Benedetti, Jed F. Fisher, Shahriar Mobashery

chapter 19|10 pages

The Human Microbiome in Health and Disease

BySandra B. Andersen, Menghan Liu, Martin J. Blaser

chapter 20|14 pages

The Phylum Actinobacteria

ByAlan C. Ward, Nagamani Bora, Jenileima Devi, Alexander Escasinas, Nicholas Allenby

chapter 21|20 pages


ByNina Dombrowski, Tara Mahendrarajah, Sarah T. Gross, Laura Eme, Anja Spang

chapter 22|30 pages

The Genus Bacillus

ByDaniel R. Zeigler, John B. Perkins

chapter 23|18 pages

The Genus Bordetella *

ByRita Austin, Tonya Shearin-Patterson

chapter 24|14 pages

The Genus Campylobacter

ByCollette Fitzgerald, Janet Pruckler, Maria Karlsson, Patrick Kwan, Janet Pruckler, Lavin Joseph, Hayat Caidi, Mark Laughlin, Rachael D. Aubert

chapter 25|14 pages


ByLourdes G. Bahamonde

chapter 26|14 pages

The Genus Clostridium

ByPeter Dürre

chapter 27|14 pages

The Genus Corynebacterium

ByLothar Eggeling, Michael Bott

chapter 28|10 pages

The Family Enterobacteriaceae

ByJ. Michael Janda, Denise L. Lopez

chapter 29|12 pages

Haemophilus Species

ByElisabeth Adderson

chapter 30|24 pages

The Genus Helicobacter

ByErnestine M. Vellozzi, Edmund R. Giugliano

chapter 31|12 pages

The Genus Legionella

ByAshley M. Joseph, Stephanie R. Shames

chapter 32|32 pages

The Genus Listeria

BySukhadeo Barbuddhe, Torsten Hain, Swapnil P. Doijad, Trinad Chakraborty

chapter 33|22 pages

The Genus Mycobacterium

ByLeen Rigouts, Sari Cogneau
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chapter 34|22 pages

Mycoplasma and Related Organisms

ByBahman Rostama, Meghan A. May

chapter 35|6 pages

The Family Neisseriaceae

ByYvonne A. Lue

chapter 36|18 pages

The Genus Pseudomonas

ByLayla Ramos-Hegazy, Shubham Chakravarty, Gregory G. Anderson

chapter 37|16 pages

The Family Rickettsiaceae

ByTimothy P. Driscoll, Victoria I. Verhoeve, Magda Beier-Sexton, Abdu F. Azad, Joseph J. Gillespie

chapter 38|16 pages

Microbiological and Clinical Aspects of the Pathogenic Spirochetes

ByCharles S. Pavia

chapter 39|24 pages

Staphylococcus aureus and Related Staphylococci

ByVolker Winstel, Olaf Schneewind, Dominique Missiakas

chapter 40|12 pages


ByVincent A. Fischetti, Patricia Ryan

chapter 41|8 pages

The Genus Vibrio and Related Genera

BySeon Young Choi, Anwar Huq, Rita R. Colwell

chapter 42|8 pages


ByRyan F. Relich, Meghan A. May

chapter 44|6 pages

Other Gram-Negative Bacteria: Acinetobacter, Burkholderia, and Moraxella

ByRebecca E. Colman, Jason W. Sahl

chapter 45|12 pages

Selected Zoonotic Pathogens

BySanjay K. Shukla, Steven L. Foley

chapter 46|34 pages


ByCharles Adair

chapter 47|18 pages

Introduction to Parasites

ByPurnima Bhanot

chapter 48|20 pages

Introduction to Bacteriophages

ByElizabeth Kutter, Emanuel Goldman

chapter 49|20 pages

Introduction to Virology

ByKen S. Rosenthal

chapter 50|22 pages

Emerging Viruses

ByMeghan A. May, Ryan F. Relich

part Part III|162 pages

Applied Practical Microbiology

chapter 74651|30 pages

Mechanisms of Action of Antibacterial Agents

ByAmmara Mushtaq, Joseph Adrian L. Buensalido, Carmen E. DeMarco, Rimsha Sohail, Stephen A. Lerner

chapter 52|12 pages

Mechanisms of Action of Antifungal Agents

ByJeffrey M. Rybak, P. David Rogers

chapter 53|12 pages

Mechanisms of Action of Antiviral Agents

ByGuido Antonelli, Francesca Falasca, Ombretta Turriziani

chapter 54|24 pages

Phage Therapy: Bacteriophages as Natural, Self-Replicating Antimicrobials

ByNaomi Hoyle, Elizabeth Kutter

chapter 55|16 pages

Emergence of Antimicrobial Resistance in Hospitals

ByParamita Basu, Joshua Garcia, Priyank Kumar

chapter 56|16 pages

Emerging Antimicrobial-Resistant Microorganisms in the Community

ByNegin Alizadeh Shaygh, Divya Sarvaiya, Paramita Basu

chapter 57|16 pages

Overview of Biofilms and Some Key Methods for Their Study

ByParamita Basu, Michael Boadu, Irvin N. Hirshfield

chapter 58|6 pages

Biofilms in Healthcare

ByRebecca K. Kavanagh, Arindam Mitra, Paramita Basu

chapter 59|8 pages

The Business of Microbiology

ByMichael C. Nugent, Lorrence H. Green

chapter 60|4 pages

Launching a Microbiology-Based Company

ByLeonard Osser

chapter 61|8 pages

Microbiology for Dental Hygienists

ByVictoria Benvenuto, Donna L Catapano

chapter 62|6 pages

Microbiology for Pre-College Teachers

ByMadge Nanney, Scott Sowell

chapter 63|2 pages

Microbiology for Home Inspectors

ByWilliam E. Herrmann