This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

section I|162 pages

Advanced methods of analysis

chapter 1|22 pages

Confocal microscopy in the life sciences

ByMiguel Aroso, M. Gomez-Lazaro

chapter 2|14 pages

Imaging flow cytometry for quantification of cellular parameters

ByCatarina R. Almeida

chapter 3|9 pages

Live-cell imaging

Seeing is believing
ByPatrícia Castro, Patrícia Carneiro

chapter 4|16 pages

Atomic force microscopy

A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems
ByCatarina S. Lopes, Filomena A. Carvalho, Nuno C. Santos

chapter 5|19 pages

Confocal Raman microscopy

Imaging the chemistry
ByM. Gomez-Lazaro, A. Freitas, C.C. Ribeiro

chapter 6|19 pages

Tracking cancer In vivo imaging techniques

ByPatrícia Carvalho, André Roma, Sérgia Velho

chapter 7|14 pages

Quantum dots

Concepts, imaging, and therapeutic applications in cancer
ByMaria Sofia Fernandes, Anabela Ferro, Patrícia Carneiro, Raquel Seruca, João M. Sanches

chapter 8|14 pages

Selecting the appropriate in situ proximity ligation assay protocol

ByDoroteya Raykova, Linda Arngården, Axel Klaesson, Johan Heldin, Ola Söderberg

chapter 9|30 pages

Cell detection and joint shape tracking using local image filters

ByTiago Esteves, Pedro Quelhas

section II|153 pages

Molecular and cellular applications

chapter 10|15 pages

Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors

ByRenato Socodato, Camila C. Portugal, Paula Sampaio, João B. Relvas

chapter 11|20 pages

Membrane trafficking under the microscope, what new imaging technologies have brought to light

ByJulia Fernandez-Rodriguez, Tommy Nilsson

chapter 12|30 pages

Illuminating the cycle of life

ByAnabela Ferro, Patrícia Carneiro, Maria Sofia Fernandes, Tânia Mestre, Ivan Sahumbaiev, João M. Sanches, Raquel Seruca

chapter 13|27 pages

Methods for the visualization of circadian rhythms: From molecules to organisms

ByRukeia El-Athman, Jeannine Mazuch, Luise Fuhr, Mónica Abreu, Nikolai Genov, Angela Relógio

chapter 14|20 pages

Measurement of lysosomal ion homeostasis by fluorescence microscopy

ByBenjamin König, Lisa von Kleist, Tobias Stauber

chapter 15|19 pages

Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations

ByJoana Figueiredo, Ana Sofia Ribeiro, Tânia Mestre, Sofia Esménio, Martina Fonseca, Joana Paredes, Raquel Seruca, João M. Sanches

chapter 16|17 pages

Cancer cell invadopodia

Visualization and quantification tools
ByAngela Margarida Costa, Maria José Oliveira