Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

chapter 1|24 pages

Electrophysiological Methods for Recording CRAC and TRPV5/6 Channels

ByJ. Ashot Kozak, Grigori Rychkov

chapter 2|26 pages

Studies of Structure–Function and Subunit Composition of Orai/STIM Channel

ByMarc Fahrner, Rainer Schindl, Christoph Romanin

chapter 3|22 pages

Signaling ER Store Depletion to Plasma Membrane Orai Channels

ByAparna Gudlur, Patrick Hogan

chapter 4|20 pages

Modulation of Orai1 and STIM1 by Cellular Factors

ByJin Seok Woo, Sonal Srikanth, Yousang Gwack

chapter 5|14 pages

CRAC Channels and Ca2+-Dependent Gene Expression

ByYi-Chun Yeh, Anant B. Parekh

chapter 6|20 pages

Function of Orai/Stim Proteins Studied in Transgenic Animal Models

ByMasatsugu Oh-Hora, Xiuyuan Lu

chapter 7|18 pages

Assessing the Molecular Nature of the STIM1/Orai1 Coupling Interface Using FRET Approaches

ByYandong Zhou, Youjun Wang, Donald L. Gill

chapter 8|16 pages

Optogenetic Approaches to Control Calcium Entry in Non-Excitable Cells

ByLian He, Qian Zhang, Yubin Zhou, Yun Huang

chapter 9|16 pages

Regulation of Orai/STIM Channels by pH

ByAlbert S. Yu, Zhichao Yue, Jianlin Feng, Lixia Yue

chapter 10|20 pages

Non-Orai Partners of STIM Proteins

Role in ER–PM Communication and Ca2+ Signaling
ByKlaus Groschner, Niroj Shrestha, Nicola Fameli

chapter 11|18 pages

Store-Independent Orai Channels Regulated by STIM

ByXuexin Zhang, Maxime Gueguinou, Mohamed Trebak

chapter 12|26 pages

Regulation and Role of Store-Operated Ca2+ Entry in Cellular Proliferation

ByRawad Hodeify, Fang Yu, Raphael Courjaret, Nancy Nader, Maya Dib, Lu Sun, Ethel Adap, Satanay Hubrack, Khaled Machaca

chapter 13|34 pages

TRPV5 and TRPV6 Calcium-Selective Channels

ByJi-Bin Peng, Yoshiro Suzuki, Gergely Gyimesi, Matthias A. Hediger

chapter 14|18 pages

Determining the Crystal Structure of TRPV6

ByKei Saotome, Appu K. Singh, Alexander I. Sobolevsky

chapter 15|18 pages

Identifying TRP Channel Subunit Stoichiometry Using Combined Single Channel Single Molecule Determinations (SC-SMD)

ByLaura G. Ceballos, Alexander Asanov, Luis Vaca

chapter 16|14 pages

Pharmacology of Store-Operated Calcium Entry Channels

ByGary S. Bird, James W. Putney