The use of light for probing and imaging biomedical media is promising for the development of safe, noninvasive, and inexpensive clinical imaging modalities with diagnostic ability. The advent of ultrafast lasers has enabled applications of nonlinear optical processes, which allow deeper imaging in biological tissues with higher spatial resolution. This book provides an overview of emerging novel optical imaging techniques, Gaussian beam optics, light scattering, nonlinear optics, and nonlinear optical tomography of tissues and cells. It consists of pioneering works that employ different linear and nonlinear optical imaging techniques for deep tissue imaging, including the new applications of single- and multiphoton excitation fluorescence, Raman scattering, resonance Raman spectroscopy, second harmonic generation, stimulated Raman scattering gain and loss, coherent anti-Stokes Raman spectroscopy, and near-infrared and mid-infrared supercontinuum spectroscopy. The book is a comprehensive reference of emerging deep tissue imaging techniques for researchers and students working in various disciplines.

chapter 1|30 pages

Overview of Second- and Third-Order Nonlinear Optical Processes for Deep Imaging

ByMurugkar Sangeeta, Robert W. Boyd

chapter 2|50 pages

Complex Light Beams

ByEnrique J. Galvez

chapter 3|10 pages

Gaussian Beam Optical Parameters in Multi-Photon Excitation Fluorescence Imaging

ByShi Lingyan, Rodríguez-Contreras Adrián, Robert R. Alfano

chapter 4|16 pages

The Optics of Deep Optical Imaging in Tissues Using Long Wavelengths

BySteven L. Jacques

chapter 5|50 pages

Light Propagation and Interaction in Highly Scattering Media for Deep Tissue Imaging

ByW. B. Wang, Shi Lingyan, Lu Luyao, Laura A. Sordillo, L. Wang, S.K. Gayen, R.R. Alfanoa

chapter 6|42 pages

Application of Nonlinear Microscopy in Life Sciences

ByZdenek Svindrych, Ammasi Periasamy

chapter 9|28 pages

Light Propagation in Turbid Tissue-Like Scattering Media

ByBykov Alexander, Doronin Alexander, Igor Meglinski

chapter 12|36 pages

Terahertz Propagation in Tissues and Its Thickness Limitation

ByKaragoz Burcu, Hakan Altan

chapter 13|18 pages

Detection of Brain Tumors Using Stimulated Raman Scattering Microscopy

ByLewis Spencer, Orringer Daniel

chapter 15|31 pages

Deep Tissue Imaging: Acoustic and Thermal Wave Propagation and Light Interactions in Tissue

BySteinberg Idan, Shoval Asaf, Balberg Michal, Sheinfeld Adi, Tepper Michal, Gannot Israel

chapter 16|28 pages

Using the Transmission Matrix to Image Disordered Media

ByDavy Matthieu, Gigan Sylvain, Azriel Z. Genack