Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.

chapter 1|14 pages


ByR. E. F. Matthews

chapter 2|34 pages

Host Plants in Diagnosis

ByJ. Horváth

chapter 3|24 pages

Experimental Transmission of Viruses in Diagnosis

ByA. T. Jones

chapter 5|28 pages

Inclusions in Diagnosing Plant Virus Diseases

ByJ. R. Edwardson, R. G. Christie, D. E. Purcifull, M. A. Petersen

chapter 6|30 pages

Virus Purification in Relation to Diagnosis

ByRichard Stace-Smith, Robert R. Martin

chapter 7|56 pages

Serological Procedures

ByM.H.V. Van Regenmortel, M.-C. Dubs

chapter 8|38 pages

Electron Microscopy of In Vitro Preparations

ByRobert G. Milne

chapter 9|20 pages

Nucleic Acid Hybridization Procedures

ByRoger Hull

chapter 10|22 pages

dsRNA in Diagnosis

ByJ. A. Dodds

chapter 11|20 pages

Diagnostic Methods Applicable to Viroids

ByD. Hanold

chapter 13|26 pages

Complexes of Transmission-Dependent and Helper Viruses

ByA. F. Murant