ABSTRACT
This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems use most frequently, including all three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of a dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. The reader will note that in this section, as well as in most of the rest of the volume, the orientation is primarily biochemical, biologic, and medical, rather than pure chemistry or pure physics. Nevertheless, the techniques are widely applicable to a variety of disciplines.
TABLE OF CONTENTS
part |2 pages
Preparative Procedures
part |2 pages
Oxygen Radical Generating Systems
part |2 pages
Analytical Methods
part |2 pages
Measurement of Superoxide Anion
part |2 pages
Detection of Hydroxyl Radical
part |2 pages
Other Radical Systems
part |2 pages
Quantitation of Superoxide Dismutase
part |2 pages
Tissue Assays
part |2 pages
Cellular Phenomenon
part |2 pages
Miscellaneous Methods