Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

chapter 1|24 pages

Isolation and Purification of DNA

chapter 2|20 pages

Isolation and Purification of RNA

chapter 3|22 pages

Extraction and Purification of Proteins

chapter 4|18 pages

Prepatation of Nucleic Acid Probes

chapter 5|20 pages

Southern Blo Hybridization

chapter 6|8 pages

Northern Blot Hybridization

chapter 8|20 pages

Western Blot Hybridization

chapter 9|46 pages

cDNA Libraries

chapter 10|44 pages

Genomic DNA Libraries

chapter 11|34 pages

DNA Sequencing and Analysis

chapter 12|20 pages

DNA Site-Directed and Deletion Mutagenesis

chapter 13|14 pages

DNA Footprinting and Gel-Retardation Assay

chapter 14|14 pages

Differential Display

chapter 17|20 pages

Plant Tissue and Cell Culture

chapter 18|32 pages

Gene Transfer and Expression in Animals

chapter 19|24 pages

Gene Transfer and Expression in Plants

chapter 20|28 pages

Inhibition of Gene Expression

chapter 24|14 pages

Combinatorial Techniques