A thorough overview of nanobiotechnology and its place in advances in applied science and engineering, The Nanobiotechnology Handbook combines contributions from physics, bioorganic and bioinorganic chemistry, molecular and cellular biology, materials science, and medicine as well as from mechanical, electrical, chemical, and biomedical engineering to address the full scope of current and future developments. World-class experts discuss the role of nanobiotechnology in bioanalysis, biomolecular and biomedical nanotechnology, biosensors, biocatalysis and biofuel, and education and workforce development. It includes downloadable resources that contain all figures in the book.

The book begins with discussions of biomimetic nanotechnology, including a comprehensive overview of DNA nanostructure and DNA-inspired nanotechnology, aptamer-functionalized nanomaterials as artificial antibodies, artificial enzymes, molecular motors, and RNA structures and RNA-inspired nanotechnology. It shows how nanotechnology can be inspired by nature as well as adverse biological events in diagnostic and therapeutic development. From there, the chapters cover major important and widely used nanofabrication techniques, applications of nanotechnology for bioprocessing followed by coverage of the applications of atomic force microscopy (AFM), optical tweezers and nanofluidics as well as other nanotechnology-enabled biomolecular and cellular manipulation and detection.

Focusing on major research trends, the book highlights the importance of nanobiotechnology to a range of medical applications such as stem cell technology and tissue engineering, drug development and delivery, imaging, diagnostics, and therapeutics. And with coverage of topics such as nanotoxicity, responsible nanotechnology, and educational and workforce development, it provides a unique overview and perspective of nanobiotechnology impacts from a researcher’s, entrepreneur’s, economist’s and educator’s point of view. It provides a resource for current applications and future development of nanobiotechnology.

part I|240 pages

Biomimetic Nanotechnology

chapter 1|28 pages

DNA Nanostructures

ByMarya Lieberman

chapter 2|15 pages

Aptamer-Functionalized Nanomaterials for Cell Recognition

ByJing Zhou, Yong Wang

chapter 3|25 pages

Artificial Enzymes

ByJames A. Stapleton, Agustina Rodriguez-Granillo, Vikas Nanda

chapter 4|40 pages

Molecular Motors

ByTimothy D. Riehlman, Zachary T. Olmsted, Janet L. Paluh

chapter 5|14 pages

From RNA Structures to RNA Nanomachines

BySabarinath Jayaseelan, Paul D. Kutscha, Francis Doyle, Scott A. Tenenbaum

chapter 6|17 pages

DNA Damage Response Research, Inherent and Future Nano-Based Interfaces for Personalized Medicine

ByMadhu Dyavaiah, Lauren Endres, Yiching Hsieh, William Towns, Thomas J. Begley

chapter 7|36 pages

Virus-Based Nanobiotechnology

ByMagnus Bergkvist, Brian A. Cohen

chapter 8|22 pages

Biomimetic Nanotopography Strategies for Extracellular Matrix Construction

ByEsther J. Lee, Kam W. Leong

chapter 9|20 pages

Butterfly Wing–Inspired Nanotechnology

ByRajan Kumar, Sheila Smith, James McNeilan, Michael Keeton, Joseph Sanders, Alexander Talamo, Christopher Bowman, Yubing Xie

part II|80 pages


chapter 11|16 pages

Microcontact Printing

ByJingjiao Guan

chapter 12|14 pages

Electron Beam Lithography for Biological Applications

ByJohn G. Hartley

chapter 13|20 pages

Laser Direct-Write

ByTimothy Krentz, Theresa Phamduy, Brian Riggs, Brian Ozsdolay, Douglas B. Chrisey

chapter 14|28 pages

Electrospinning of Nanofibers

ByAndrea M. Unser, Yubing Xie

part III|45 pages


chapter 15|43 pages

Applications of Nanotechnology to Bioprocessing

BySusan T. Sharfstein, Sarah Nicoletti

part IV|131 pages

Biomolecular and Cellular Manipulation and Detection

chapter 16|23 pages

Atomic Force Microscopy

ByGunjan Agarwal, Tanya M. Nocera

chapter 17|29 pages


ByShiqing Wu, Shengnian Wang

chapter 18|16 pages


ByXin Hu, Weixiong Wang

chapter 19|22 pages

Optical Tweezers

ByYingbo Zu, Fangfang Ren, Shengnian Wang

chapter 20|25 pages

Cellular Response to Nanoscale Features

ByManus J.P. Biggs, Matthew J. Dalby, Shalom J. Wind

chapter 21|11 pages

Micro- and Nanotechnologies in Integrative Biology

ByXulang Zhang

part V|97 pages

Biomedical Nanotechnology

chapter 22|18 pages

Micro- and Nanotechnology in Tissue Engineering

ByJane Wang, Robert Langer, Jeffrey T. Borenstein

chapter 23|16 pages

Nanotechnology in Drug Delivery

ByJungmin Cho, Sungwon Kim, Kinam Park

chapter 24|17 pages

Lipid-Based Nanoparticles for siRNA Delivery

ByBo Yu, L. James Lee, Robert J. Lee

chapter 25|27 pages

Nanodiamonds for Bioimaging and Therapeutic Applications

ByV. Vaijayanthimala, Yuen Yung Hui, Huan-Cheng Chang

chapter 26|15 pages

Biomedical Micro Probe for Super Resolved Image Extraction

ByAsaf Shahmoon, Shiran Aharon, Dror Fixler, Hamutal Slovin, Zeev Zalevsky

part VI|52 pages

Nanobiotechnology Impacts

chapter 27|22 pages


ByRui Chen, Chunying Chen

chapter 28|8 pages

Responsible Nanotechnology: Controlling Exposure and Environmental Release via Rational Design

ByNathaniel C. Cady, Aaron D. Strickland

chapter 29|20 pages

Educational and Workforce Development in Nanobiotechnology

ByLaura I. Schultz, Daniel D. White