This book integrates recent advances in molecular and cell biology of hematopoietic stem cells (HSC) with developments in clinical research in stem cell-based therapy-providing an up-to-date review of novel cytokines and cellular components; animal models; cell preparation, selection, and collection; minimal residual disease and purging; expansion

chapter 1|21 pages

Thrombopoietin: Basic Biology and Clinical Effects

ByKenneth Kaushansky, C. Glenn Begley

chapter 2|7 pages

Preclinical Biology and Potential Clinical Utility of flt3 Ligand

ByDouglas E. Williams

chapter 3|16 pages

Stem Cell Factor

ByLeo Lacerna, Jr., Russell Basser, C. Glenn Begley, Jeffrey Crawford, George Demetri, Craig H. Moskowitz, Guido Tricot, Andrew Weaver, William P. Sheridan, and John A. Glaspy

chapter 4|22 pages

Cytokine Modulation of Hematopoietic Stem Cell Phenotype

ByAhmad-Samer Al-Homsi, Peter J. Quesenberry

chapter 5|17 pages

Homing and Self-Renewal of Hematopoietic Stem Cells

ByAnthony D. Ho, Michael Punzel, Stefan Fruehauf, Shiang Huang, Bernhard Palsson

chapter 6|24 pages

Requirements for Hematopoietic Stem Cell Engraftment and Graft Engineering: Role of Facilitating Cells

ByMichael Neipp, Christina L. Kaufman, Marianne Bergheim, Beate G. Exner, Suzanne T. Ildstad

chapter 7|14 pages

Ins and Outs of Hematopoietic Stem Cells: Regulation by Genes and Stromal Cells

ByChrista E. Mu¨ller-Sieburg and Rajeev Soni

chapter 8|12 pages

Cell-Signaling by Adhesion Receptors in Normal and Leukemic Hematopoiesis

ByCatherine M. Verfaillie, Yuehua Jiang, Robert C. H. Zhao, Felipe Prosper, Ravi Bhatia

chapter 9|16 pages

Detection of Infrequent Cells in Blood and Bone Marrow by Flow Cytometry

ByLeon W. M. M. Terstappen

chapter 10|18 pages

Human/Sheep Hematopoietic Chimeras

chapter 11|24 pages

Canine Models for Transplantation and Gene Therapy

ByHans-Peter Kiem, Rainer Storb

chapter 12|28 pages

Murine Model of In Utero Transplantation

ByEwa Carrier

chapter 13|15 pages

CD34 Selection Using Immunomagnetic Beads

ByRobert A. Preti, Andrew L. Pecora, Tauseef Ahmed, Timothy J. Farley

chapter 14|11 pages

Autologous and Allogeneic Transplantation with Blood CD34 Cells: A Pediatric Experience

ByYoshifumi Kawano, Yoichi Takaue, Tsutomu Watanabe, Yasuhiro Okamoto, Takanori Abe, Yasuhiro Kuroda, Arata Watanabe

chapter 15|23 pages

Using Allogeneic Graft Engineering to Improve Long-Term Survival

ByStephen J. Noga

chapter 17|12 pages

Stem Cell Isolation with Immunomagnetic Beads and Tumor Cell Contamination

ByGunnar Kvalheim, Anne Pharo, Harald Holte, Elisabeth Lenschow, Mengyu Wang, Bjorn Erikstein, Stein Kvaloy, John Magne Nesland, Erlend Smeland

chapter 18|17 pages

CD34 Cell Culture: Clinical Needs and Applications

ByDennis E. Van Epps

chapter 19|11 pages

Critical Parameters Influencing the Expansion and Differentiation of Cultured Human CD34 Cells

ByJane S. Lebkowski, Lisa R. Schain

chapter 20|28 pages

Mobilization of Peripheral Blood Stem Cells from Normal Donors

ByThomas A. Lane, Ping Law

chapter 22|20 pages

Peripheral Blood Stem Cells for Allogeneic Transplantation

ByLutz Uharek, Peter Dreger, Bertram Glass, Matthias Zeis, Norbert Schmitz

chapter 23|10 pages

Mechanisms of Cure of Acute Myeloid Leukemia with Allogeneic Transplantation

ByFrederick R. Appelbaum

chapter 24|21 pages

Late Complications of Hematopoietic Stem Cell Transplantation

ByEmin Kansu, Keith M. Sullivan

chapter 25|12 pages

Prevention of Acute Graft-Versus-Host Disease by Delayed or Selected Lymphocyte Add Back

ByJohn Barrett, Dimitrios Mavroudis

chapter 27|22 pages

Autologous Peripheral Blood Stem Cell Collection and Engraftment

ByStefan Hohaus, Maria Teresa Voso, Simona Martin, Rainer Haas

chapter 28|19 pages

Stem Cell Transplantation for Hodgkin’s Disease

ByRam Kancherla, Tauseef Ahmed

chapter 29|18 pages

Stem Cell Transplantation for Multiple Myeloma—10 Years Later

ByDavid S. Siegel, Seah H. Lim, Guido Tricot, K. R. Desikan, A. Fassas, Jayesh Mehta, Seema Singhal, Elias Anaissie, Sundar Jagannath, Barthel Barlogie

chapter 30|20 pages

High-Dose Therapy for Primary and Metastatic Breast Cancer

ByGary Spitzer, Kenneth Meehan, Douglas Adkins

chapter 31|16 pages

Gene Therapy Using Hematopoietic Stem Cells

ByDonald B. Kohn, Gay M. Crooks, Jan A. Nolta

chapter 32|16 pages

Gene Therapy for Human Immunodeficiency Virus Infection Using Stem Cell Transplantation

ByAlain Gervaix, Flossie Wong-Staal

chapter 34|19 pages

Development of Retroviral Vectors that Target Hematopoietic Stem Cells

ByCells Chris A. Benedict, Yi Zhao, Nori Kasahara, Paula M. Cannon, and W. French Anderson