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Book

Microarray Technology Through Applications

Book

Microarray Technology Through Applications

DOI link for Microarray Technology Through Applications

Microarray Technology Through Applications book

Microarray Technology Through Applications

DOI link for Microarray Technology Through Applications

Microarray Technology Through Applications book

Edited ByFrancesco Falciani
Edition 1st Edition
First Published 2007
eBook Published 30 March 2007
Pub. Location London
Imprint Taylor & Francis
DOI https://doi.org/10.4324/9780203967270
Pages 276
eBook ISBN 9780203967270
Subjects Bioscience
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Falciani, F. (Ed.). (2007). Microarray Technology Through Applications (1st ed.). Taylor & Francis. https://doi.org/10.4324/9780203967270

ABSTRACT

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology.

The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation.

Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536

TABLE OF CONTENTS

chapter 1|52 pages

Introduction to microarray technology

Edited ByFrancesco Falciani

chapter 2|20 pages

Immunoprecipitation with microarrays to determine the genome-wide binding profile of a DNA-associated protein

ByJoseph T. Wade

chapter 3|16 pages

Array-based comparative genomic hybridization as a tool for solving practical biological and medical questions

ByDavid Blesa, Sandra Rodríguez-Perales, Sara Alvarez, Cristina Largo, Juan C. Cigudosa

chapter 4|20 pages

Use of single nucleotide polymorphism arrays: Design, tools, and applications

ByMercedes Robledo, Anna González-Neira, Joaquín Dopazo

chapter 5|16 pages

In vitro analysis of gene expression

ByDonling Zheng, Chrystala Constantinidou, Jon L. Hobman, Steve D. Minchin

chapter 6|30 pages

The analysis of cellular transcriptional response at the genome level: Two case studies with relevance to bacterial pathogenesis

ByThomas Carzaniga, Donatella Sarti, Victor Trevino, Christopher Buckley, Mike Salmon, Shabnam Moobed, David Wild, Chrystala Constantinidou, Jon L. Hobman, Gianni Dehò, Francesco Falciani

chapter 7|18 pages

Functional annotation of microarray experiments

ByJoaquín Dopazo, Fátima Al-Shahrour

chapter 8|38 pages

Microarray technology in agricultural research

ByAna Conesa, Javier Forment, José Gadea, Jeroen van Dijk

chapter 9|16 pages

Protein microarrays

ByNigel J. Saunders

chapter |4 pages

SECTION 2: EXPERIMENTAL PROTOCOLS

Edited ByFrancesco Falciani

chapter |4 pages

Protocol 2: Extraction of E. coli RNA

Edited ByFrancesco Falciani

chapter |6 pages

Protocol 3: Probe labeling

Edited ByFrancesco Falciani

chapter |6 pages

Protocol 4: Hybridization and washing

Edited ByFrancesco Falciani

chapter |2 pages

Protocol 5: ChIP procedure

Edited ByFrancesco Falciani

chapter |4 pages

Protocol 6: Array comparative genomic hybridization (CGH)

Edited ByFrancesco Falciani

chapter |6 pages

Protocol 7: Run-off microarray analysis of gene expression (ROMA)

Edited ByFrancesco Falciani

chapter |2 pages

Protocol 8: Selection and genotyping of single nucleotide polymorphisms

Edited ByFrancesco Falciani
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