ABSTRACT
The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.
TABLE OF CONTENTS
part I|2 pages
Immunocytomas in LOUVAIN (LOU) Rats
part II|8 pages
Production of Rat-Rat Hybridomas
part III|2 pages
Production, Purificaton, Fragmentation, and Labeling of Rat Monoclonal Antibodies
part IV|2 pages
Examples of Production and Characterization of Rat-Rat Hybridomas Against Various Antigens
chapter 21.I|4 pages
Rat Monoclonal Antibodies Against Streptococcus Mutans Anobacteroides Gingivalis
chapter 21.II|8 pages
A Specific rat Monoclonal Antibody Against Yersinia Enterocolit1Ca Serogroup 0:8
part V|2 pages
Specific Uses and Properties of Rat Monoclonal Antibodies