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      Sedimentation Velocity Analytical Ultracentrifugation
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      Book

      Sedimentation Velocity Analytical Ultracentrifugation

      DOI link for Sedimentation Velocity Analytical Ultracentrifugation

      Sedimentation Velocity Analytical Ultracentrifugation book

      Discrete Species and Size-Distributions of Macromolecules and Particles

      Sedimentation Velocity Analytical Ultracentrifugation

      DOI link for Sedimentation Velocity Analytical Ultracentrifugation

      Sedimentation Velocity Analytical Ultracentrifugation book

      Discrete Species and Size-Distributions of Macromolecules and Particles
      ByPeter Schuck
      Edition 1st Edition
      First Published 2016
      eBook Published 9 November 2016
      Pub. Location Boca Raton
      Imprint CRC Press
      DOI https://doi.org/10.1201/9781315367231
      Pages 266
      eBook ISBN 9781315367231
      Subjects Bioscience, Physical Sciences
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      Get Citation

      Schuck, P. (2016). Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles (1st ed.). CRC Press. https://doi.org/10.1201/9781315367231

      ABSTRACT

      A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles.

      The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems.

      Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx

      TABLE OF CONTENTS

      chapter 1|20 pages

      Basic Analysis Principles

      chapter 2|34 pages

      Sedimentation of Discrete Non-Interacting Particles

      chapter 3|22 pages

      Properties of Sedimentation Coefficient Distributions

      chapter 4|14 pages

      Distributions of Non-Diffusing Particles

      chapter 5|40 pages

      Distributions of Diffusing Particles

      chapter 6|22 pages

      Sedimentation Coefficient Distributions from Boundary Derivatives and Extrapolations

      chapter 7|18 pages

      Multi-Component Distributions

      chapter 8|38 pages

      Practical Analysis of Non-Interacting Systems

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