Asthma and Respiratory Infections

J. WITEK, Jr.

of both children and adults. of viruses was far more common during exacerbations of infection. Clarke (32) of 51 adult asthmatics, recruited from a hospital outpatient of these exacerbations and RT viruses isolated in 4 of these of 10.8%. A similar identification rate was found by Hudgel of 19 patients from a hospital clinic. Viral identification

of the replicating virus on the cell and of the host to this infection. This response has both an innate compo-

of the submucosal and adventitial extracellular of fluid exudation from the capillaries has only a small effect of the airway lumen. However, when the smooth muscle contracts,

of children who develop recurrent wheezing and of viral respiratory infection in infancy frequently show al-

I. Introduction

of nasal spray from an atomizer and/or nasal inhala- of these methods have been designed to enhance of these methods has been

of the role played

of the specimen should be made prior to storage. For antigen of 4°C (or -20°C for longer periods) is often

Detection of Viral Antigen of viruses in nasopharyngeal aspirates of patients with acute respi- of the original rapid diagnostic methods.

of a single chain of nucleotides. Viral nucleic acids are usually double-

"dot" or a "slot" for subsequent probing. This slot-blot of being semi-quantitative if a

of the PCR assay have been varied according to the application of this theme applicable to the of respiratory viruses are discussed. Nested PCR involves a second of 15-30 cycles of PCR performed on the first-round PCR product using

of PCR method, the target virus genome, and detection limits of

of a dual infection in 20% by RT-PCR compared of commercial extrac- of the PCR processes, and accessibility to virus

Rhinoviruses of rhinovirus infections has been attempted in many of the virus in 1956. In spite of the effort and