An All-Inclusive Guide to Surgical Techniques on Rats

The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.

Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.

CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction

With succinct descriptions, color photographs, and easy-to-follow steps, the downloadable resources are a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for today’s biomedical sciences investigator.

chapter |2 pages

Section I: Introduction

chapter 1|6 pages

Bioethics and Animal Care

chapter 2|8 pages

Control of Stress and Distress

chapter 3|4 pages

Management of Pain in Laboratory Animals

chapter 4|10 pages

Anesthesia and Analgesia

chapter 5|2 pages


chapter 6|2 pages


chapter |2 pages

Section II: General Procedures

chapter 7|4 pages

Operating Theatre

chapter 8|2 pages

Rat Identification

chapter 9|4 pages

Antisepsis, Sterilization, and Asepsis

chapter 10|8 pages

1Chapter 0 Suture

chapter 11|8 pages

1Chapter 1 Substances Administration

chapter 12|8 pages

1Chapter 2 Samples

chapter 14|6 pages

1Chapter 4 Cardiac Catheterization

chapter 16|2 pages

1Chapter 6 Tracheostomy

chapter |2 pages

Section IV: Gastrointestinal Tract

chapter 19|4 pages

1Chapter 9 Intestinal Everted Sacs

chapter 20|4 pages

In Situ Perfusion of the Small Intestine

chapter |2 pages

Section V: Pancreas

chapter 22|2 pages

2Chapter 2 Islets of Langerhans Isolation

chapter 25|8 pages

2Chapter 5 Experimental Pancreatitis

chapter |2 pages

Section VI: Liver

chapter 26|4 pages

2Chapter 6 Hepatic Circulation Impairment

chapter 27|4 pages

2Chapter 7 Extrahepatic Cholestasis Model

chapter 28|4 pages

2Chapter 8 Adrenalectomy

chapter 29|4 pages

2Chapter 9 Ovariectomy

chapter 30|4 pages


chapter 31|2 pages

3Chapter 1 Parathyroid Glands

chapter |2 pages

Section VIII: Kidneys

chapter 33|8 pages

3Chapter 3 Kidney Isolation and Perfusion

chapter 34|6 pages

3Chapter 4 Acute Renal Failure Models

chapter 35|6 pages

3Chapter 5 Renal Blood Flow Measurement

chapter 37|4 pages

3Chapter 7 Remnant Kidney Model

chapter |2 pages

Section IX: Circulatory System

chapter 39|4 pages

3Chapter 9 Experimental Arteriosclerosis

chapter |2 pages

Section X: Additional Techniques

chapter 41|4 pages

Polyclonal Antibodies Preparation

chapter 42|8 pages

Histological Procedures

chapter 43|4 pages

Bones and Bone Tissue

chapter |2 pages

Section XI: Miscellaneous

chapter 44|6 pages


chapter 45|2 pages

Solution Preparation