ABSTRACT
"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "
TABLE OF CONTENTS
chapter 1|14 pages
Historical and Useful Methods of Preselection and Preparative Scale Sorting
Charlotte Esser I. BACKGROUND
chapter 2|4 pages
New Approaches in Density Gradient Separation Using Colloidal Silica Gradients in the Processing of Human Hematopoietic Progenitor Cells
Peter Van Vlasselaer, Varghese C. Palathumpat, George Strang, d Michael H. Shapero
chapter 3|4 pages
Centrifugal Elutriation: A Powerful Separation Technique in Cell Biology, Immunology, and Hematology
Carl G. Figdor, Frank Preijers, Richard Huijbens, Paul Ruijs, Theo J. M. de Witte, and Willy S. Bont I. INTRODUCTION
part 111|1 pages
RECENT DEVELOPMENTS A. Flow Systems to Maintain a Constant Flow Rate
chapter 4|6 pages
Isolation, Activation, Expansion, and Gene Transduction of Cell-Based Therapeutics Using Polystyrene Immunoaffinity Devices
Jane S. Lebkowski, Dewey J. Moody, Ramila Philip, Lisa Schain, Sohel Talib, Rukmini Pennathur-Das, and Thomas B. Okarma David A. Okrongly I. INTRODUCTION
chapter 5|3 pages
The CEPRATE® SC System: Technology, Clinical Development, and Future Directions
Shelly Heimfeld, Karen Auditore-Hargreaves, Mark Benyuenes, Michael Emde, Cindy Jacobs, Mark Jones, Nicole Provost, Grant Risdon, and Joe Tarnowski I. INTRODUCTION
chapter 6|30 pages
High-Density Particles: A Novel, Highly Efficient Cell Separation Technology
Norma S. Kenyon and Camillo Ricordi John G. Gribben and Lee M. Nadler
part 7|2 pages
Antibody- and Complement-Mediated Cell Separation
part 8|1 pages
Magnetic Cell Sorting with Colloidal Superparamagnetic Particles
part |1 pages
D. Incubation with Microspheres
chapter 11|21 pages
Cell Separation Using Flow Cytometric Cell Sorting
Robert A. Hoffman and David W. Houck I. INTRODUCTION
part C|2 pages
Single-Cell Deposition
chapter 12|5 pages
Employing Surface Markers for the Selection of Transfected Cells
Gregor Siebenkotten, Ute Behrens-Jung, and Stefan Miltenyi Katja Petry
chapter 13|8 pages
CD34+ Cell Sorting and Enrichment: Applications in Blood Banking and Transplantation
Hassan T. Hassan, K. Gutensohn, A. R. Zander, and P. Kuehnl I. HUMAN HEMATOPOIETIC CD34+ CELLS
