ABSTRACT
Saffron is a precious spice which is mainly grown in Iran, India, Spain, Greece, Italy, Pakistan, Morocco, and central Asian countries. Until recently, saffron was perceived only for its value as a spice. However, with recent research findings pointing to the medicinal properties of saffron such as its antimicrobial, anticarcinogenic and antioxidan
TABLE OF CONTENTS
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CROCUS PALIASH
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was not described. Nodal tissues with their high division activity and regeneration ability could also be used as explants. Ding et al. (31) successfully obtained callus from corm tissues and regenerated seedlings in the presence of NAA and IAA. When corm parts were used, micro corms were formed on explants grown in medium containing 2, 4-D (48). Formation of callus and development of bud and seedling was reported when coconut milk together with 2, 4-D and BAP were added to growth medium of corm explants (52). The same result was obtained from corm explants in presence of 2, 4-D and ZN. Substitution of ZN with BAP led to differentiation of shoots from callus after three months (53). Plessner et al. (72) used small corms for developing shoot from their apical and lateral buds. When the small corms were treated with ethylene and their apical buds scratched, micro corms were formed in a medium containing 2, 4-D, KN and ZN (see Table 7-2 and Figure 7-6 for details). There is only one report where callus was formed from leaf explants in presence of NAA and BAP After 8 months several buds were formed that developed into shoots when minerals of MS medium were reduced to half concentration and IAA was added (49).
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Role of Indigenous Knowledge in Traditional Agriculture with Emphasis on Saffron
