ABSTRACT

This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.

part I|2 pages

Definition

part II|2 pages

Vectors and Cloning

chapter 4|4 pages

Restriction Enzymes

chapter 5|2 pages

Electrophoresis of Nucleic Acids

chapter 6|2 pages

Description of a Plasmid and a Phagemid

chapter 9|4 pages

Molecular Cloning

part III|2 pages

Labelling of Nucleic Acids and Hybridization

chapter 11|4 pages

DNA Labelling

chapter 12|4 pages

Molecular Hybridization

chapter 13|4 pages

In situ Hybridization of mRNA

part IV|2 pages

DNA Libraries and Screening

part V|2 pages

Characterization of a Gene

chapter 23|6 pages

DNA Sequencing

chapter 24|2 pages

PCR: Polymerase Chain Reaction

chapter 25|4 pages

RACE: Rapid Amplification of cDNA Ends

chapter 26|2 pages

Genome Walking by PCR

chapter 27|4 pages

RT-PCR: Reverse Transcriptase PCR

chapter 28|2 pages

In vitro Transcription

chapter 30|4 pages

Functional Analysis of Promoters

chapter 31|2 pages

Gel Retardation

chapter 32|2 pages

DNase I Footprinting

part VI|2 pages

Genetic Transformation of Eukaryotes

chapter 35|2 pages

Direct Gene Transfer by Biolistics

chapter 36|4 pages

Genetic Transformation of Animal Cells

chapter 37|4 pages

Cloning of Animals

chapter 38|2 pages

Transient Expression

part VII|2 pages

Analysis of Gene Function

chapter 39|4 pages

Recombinant Proteins

chapter 41|6 pages

Yeast two Hybrid System

chapter 42|6 pages

Site-Directed Mutagenesis

chapter 43|4 pages

Mutation Complementation in Yeast

chapter 44|4 pages

Knock-out of Genes in Yeast

chapter 45|6 pages

Gene Tagging

chapter 46|4 pages

RNA Interference

part VIII|2 pages

Polymorphism of a Genome

chapter 47|6 pages

Molecular Markers

chapter 48|4 pages

Genetic and Physical Maps

chapter 49|4 pages

PFGE: Pulse Field Gel Electrophoresis

chapter 51|2 pages

RAPD: Random Amplified Polymorphic DNA

chapter 53|6 pages

Retromarkers

chapter 56|2 pages

SNP: Single Nucleotide Polymorphism