Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

chapter 1|26 pages

Isolation and Puri cation of DNA

chapter 2|8 pages

DNA Cloning Strategies

chapter 3|20 pages

Polymerase Chain Reaction Methodologies

chapter 4|20 pages

Preparation of Nucleic Acid Probes

chapter 5|20 pages

Southern Blot Hybridization

chapter 6|44 pages

Genomic DNA Libraries

chapter 7|26 pages

DNA Sequencing and Analysis

chapter 8|20 pages

Isolation and Puri cation of RNA

chapter 9|10 pages

Real-Time PCR and qRT-PCR Methodologies

chapter 10|14 pages

Preparation of RNA Probes

chapter 11|6 pages

Northern Blot Hybridization

chapter 12|44 pages

cDNA Libraries

chapter 13|12 pages

Differential Display

chapter 14|18 pages

Localization of RNA Expression

chapter 15|22 pages

Isolation and Puri cation of Proteins

chapter 16|18 pages

Western Blot Hybridization

chapter 17|6 pages

Localization of Protein Expression

chapter 18|14 pages

DNA Footprinting and Gel Retardation Assay

chapter 20|14 pages

Isolation and Puri cation of Metabolites

chapter 21|28 pages

Analysis of Metabolites

chapter 22|28 pages

Bioinformatics and Data Analysis

chapter 23|10 pages

Microarray Platforms

chapter 24|8 pages

Microarray Data Collection

chapter 25|4 pages

Microarray Data Analysis

chapter 26|12 pages

Proteomics: Data Collection and Analysis

chapter 27|14 pages

Metabolomics: Data Collection and Analysis

chapter 29|12 pages

Integrated Functional Genomics

chapter 30|28 pages

Inhibition of Gene Expression

chapter 31|8 pages

Inhibition of Protein Synthesis

chapter 32|22 pages

Gene Transfer and Expression in Animal Cells

chapter 35|22 pages

Plant Tissue and Cell Culture

chapter 36|28 pages

Gene Transfer and Expression in Plants

chapter 37|10 pages

Microscopy: Light and Confocal