ABSTRACT

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology.

The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation.

Colour figures and data sets are provided on the website at https://www.garlandscience.com/9780415378536

chapter 1|52 pages

Introduction to microarray technology

chapter 9|16 pages

Protein microarrays

chapter |4 pages

SECTION 2: EXPERIMENTAL PROTOCOLS

chapter |4 pages

Protocol 2: Extraction of E. coli RNA

chapter |6 pages

Protocol 3: Probe labeling

chapter |6 pages

Protocol 4: Hybridization and washing

chapter |2 pages

Protocol 5: ChIP procedure