This chapter reviews what is known about the mechanisms and significance of Ascorbate (ASC) recycling in plant and animal cells. Recycling of ASC from monodehydroascorbate (MDHA) makes sense from the viewpoint of the ASC economy of the cell, since it avoids generation of dehydroascorbate, which might ring-open or leave the cell on glucose transporters. Reduction of MDHA to ASC has also been described to occur on both the inner erythrocyte membrane and on the outer membrane surface of K562 erythroleukemic cells and human erythrocytes. Recycling of MDHA to ASC in plants is mediated by a variety of enzymatic systems in different cell compartments. MDHA in chloroplasts is mainly generated by the scavenging of hydrogen peroxide originating from the enzymatic or nonenzymatic dismutation of superoxide. The capacity for cells to recycle ASC may not only reflect their ability to combat oxidant stress, but also provide an estimate of the extent to which ASC recycling can maintain intracellular ASC homeostasis.