ABSTRACT
The challenge for constructing the functional biomimetic artificial organs in vitro paves the way for the inventions of several microfabrication technologies such as microfluidics. The most important limitations in developing artificial tissues are allowing hierarchical multi-cell interactions three-dimensionally under perfusion culture for nutrient supply and waste removal mimicking the physiological environment. By combining biology with bio-MEMS techniques, it is highly possible to overcome the above-mentioned limitations to provide a suitable environment for cells to grow and interact in vitro and mimic living systems.
