ABSTRACT
Radiosynthesis chemists are tasked with a formidable job. They must construct (synthesize) a molecule that incorporates a radioactive atom, for example, a carbon-14 (C-14) atom, at just the proper place within the molecule. Placement is crucial because some metabolic breakdown of the molecule is expected. The radioactive tag must be attached to the unmetabolized molecular component with the desired efficacious effect. The compound must be produced in a form that can be stored for long periods without molecular disintegration. The specific radioactivity (µCi per unit weight) must be well characterized. Other researchers will require this information to produce quantifiable dilutions for injections into experimental animals. The purity of the radioactive compound must be such that these subsequent in vivo experiments meet strict Food and Drug Administration guidelines. Application of “good lab practice” guidelines are required in the radiochemical laboratory to assure that calibrated equipment, reagents of certain ages and grades, and work practices meet rigorous quality standards.
