ABSTRACT
In addition to human tissues, wholemount ISH has been successfully employed for a number of experimental animal models such as embryos of mouse, Drosophila, zebrafish, or Xenopus [7-9]. Furthermore, using whole-mount ISH, large numbers of specimens can be simultaneously analyzed. Hence, this rapid method is applied to determine the individual expression pattern of randomly selected probes. Although preparation and fixation of the specimens as well as the generation of the probe must be performed manually, large-scale random screens are feasible only if the hybridization and detection procedure can also be automated. This guarantees high reproducibility of results and enough spare time for
careful recording and documentation of the proceeded specimens [10].
