ABSTRACT
Since the advent of the hybridoma technology, most monoclonal antibodies (MAbs) have been derived from the mouse species. Among the screening assays which have been developed to identify the mouse MAbs in culture supernatants, the common method utilizes the detection of antibodies capable of binding to the fixed antigen, using labeled anti-immunoglobulins (anti-Igs) as second antibodies. Nowadays, such anti-Igs reagents are important since the knowledge of Ig class and subclass is required to select the mouse MAbs for specific purposes, as complement fixation or Fc-receptors binding, 1 - 4 and to devise the strategy of their purification.
