ABSTRACT
Three-month old LOU/C rats were given two injections of 500 μg of rabbit immunoglobulin mixed with 200 μl of complete Freund’s adjuvant by the intraperitoneal route. After a rest period of 4 months, the boost of 500 μg of rabbit Ig was done 4 d before the fusion by the intravenous route (fusion made by L. De Clercq). One rat hybridoma secreting monoclonal antibodies (MAbs) against rabbit IgG was selected for its binding activity and its secretion. This MAb (LO-RG-1) is an IgG2a, and can be used to purify rabbit immunoglobulin. After peroxidase labeling, it can be very useful as second layer in immunochemistry (see Figure 5 in Chapter 24.VI).245
