ABSTRACT
LOU/C rat as wells as BALB/c mice were immunized with male Xenopus plasma from animals stimulated by 17-ß-oestradiol. Both species reacted equally well after two i.p. inoculations of 0.4 mg plasma proteins with complete Freund adjuvant at a 1 -week interval. The LOU rat chosen for fusion was boosted by i.v. inoculation of 0.4 mg plasma proteins at days 3,2, and 1 before fusion.
